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Hemp Future Week, Day 3: Cannabisin B.

Richard Rose

Your CannaCoach, Pioneer in the Hemp Industry for 24 Years. Consult the Consultant your Consultant consults.

Cannabisin B Kills Cancer

Cannabisin is a group of lignanamides found in Cannabis fruits and shells. There are 8, Cannabisin-A through -G. They may be in response to UV and image1 (1)insect predation, and are cytotoxic (like a leukocyte), anti-inflammatory, antineoplastic (anti-tumor), cause autophagy (cancer cell death), and have cardiovascular and analgesia (pain relief) activity.

But the reason Day 3 of Hemp Future Week is on Cannabisin B is not just because you’ve never heard of it, but because you’ve never heard of it and it is a cancer fighter, as well as a powerful free radical scavenger.

Shelled hempseed of course has none, although it may be in the fruits (hemp nut). Hempseed protein powder is about half granulated shells, so it’s a good source of Cannabisin B.

The economics are interesting: we now have a nontoxic cancer-fighting agent contained in a material, hempseed protein powder, which, just a few years ago, pig farmers were paid to haul away. That material is worth more than the oil which is pressed to create it, and removing the shell fragments to recover the Cannabisin B vastly improves the quality of the resulting protein powder (today protein powder is 40-70% protein, soy protein isolate is 90%). So, whereas a few years ago, oil pressing returned only the value of the oil ($5/#), today it returns the oil (~$5), a protein powder (~$5), plus now say $5 for the Cannabisin B. That’s a $15 return from a product which not long ago was returning only $5/pound. Conceivably, the cost of the actual oil should be very low since they are making their money on the by-products.

Here are two studies on Cannabisin B:

The isolation and identification of two compounds with predominant radical scavenging activity in hempseed (seed of Cannabis sativa L.)

Forty samples were extracted from defatted kernels and hulls of two varieties of hempseed (Bama and Yunma No. 1) using 10 different polar solvent systems. The radical scavenging capacity of the extracts was evaluated using 2,2-diphenyl-1-pikrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays and the total phenolic content was determined by Folin-Ciocalteu’s phenol reagent. The correlation analysis indicated that the antioxidants in hempseed belonged to phenolic and DPPH assay was suitable for evaluating the radical scavenging activity. Two compounds, with predominant antiradical activity, were isolated in 60% ethanol extract of hempseed hull using macroporous resin absorption, LH-20 gel chromatography, and high performance liquid chromatography methods, which were identified as N-trans-caffeoyltyramine and cannabisin B by high-resolution mass spectra, nuclear magnetic resonance spectra, and ultraviolet data. The two compounds exhibited significant high DPPH scavenging activity and protective effect against in vitro oxidation of human low-density lipoprotein compared with extracts from flaxseed, grape seed, and soybean. This suggests that hempseed hull extract is a potential source of natural antioxidants, which could be added to dietary supplements to help prevent oxidative stress.” Food Chem. 2012 Sep 15; 134(2):1030-7

Cannabisin B induces autophagic cell death by inhibiting the AKT/mTOR pathway and S phase cell cycle arrest in HepG2 cells

This study investigates the anticancer properties of cannabisin B, purified from hempseed hull, in HepG2 human hepatoblastoma cells. The results indicate that cannabisin B significantly inhibited cell proliferation by inducing autophagic cell death rather than typical apoptosis. Cell viability transiently increased upon the addition of a low concentration of cannabisin B but decreased upon the addition of high concentrations. Cannabisin B-induced changes in cell viability were completely inhibited by pre-treatment with 3-methyladenine (3-MA), indicating that the induction of autophagy by cannabisin B caused cell death. Additionally, cannabisin B induced S phase cell cycle arrest in a dose-dependent manner. Moreover, cannabisin B was found to inhibit survival signaling by blocking the activation of AKT and down-stream targets of the mammalian target of rapamycin (mTOR). These findings suggest that cannabisin B possesses considerable antiproliferative activity and that it may be utilised as a promising chemopreventive agent against hepatoblastoma disease. Food Chemistry, Volume 138, Issues 2–3, 1 June 2013, Pages 1034–1041″

Tomorrow on Hemp Future Week, Day 4: Cannaceuticals and Cannabiotics.

Hemp Future Week is a service of the American Hemp Association and the Medicinal Hemp Association.

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